daf-16;tub-1

Forkhead box protein O;hypothetical protein

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Locus: CELE_R13H8.1

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Wormbase description: daf-16 encodes the sole C. elegans forkhead box O (FOXO) homologue; DAF-16 functions as a transcription factor that acts in the insulin/IGF-1-mediated signaling (IIS) pathway that regulates dauer formation, longevity, fat metabolism, stress response, and innate immunity; DAF-16 regulates these various processes through isoform-specific expression, isoform-specific regulation by different AKT kinases, and differential regulation of target genes; DAF-16 can interact with the CBP-1 transcription cofactor in vitro, and interacts genetically with other genes in the insulin signaling and with daf-12, which encodes a nuclear hormone receptor; DAF-16 is activated in response to DNA damage during development and co-regulated by EGL-27, alleviates DNA-damage-induced developmental arrest by inducing DAF-16-associated element (DAE)-regulated genes; DAF-16 is broadly expressed but displays isoform-specific tissue enrichment; DAF-16 localizes to both the cytoplasm and the nucleus, with the ratio between the two an important regulator of function.

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Tubby protein homolog 1

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Locus: CELE_F10B5.4

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Wormbase description: tub-1 encodes a TUBBY homolog that affects fat storage across species including C. elegans; tub-1 functions with kat-1, a 3-ketoacyl-coA thiolase, to affect lipid accumulation; tub-1 is also required for normal life span as tub-1 mutants show extended life span which is dependent on daf-16/FOXO3a; tub-1 interacts with a novel RabGTPase-activating protein RBG-3 and upregulates the activity of a small GTPase, RAB-7, suggesting the involvement of an endocytic pathway in regulating fat storage; tub-1 is expressed in ciliated neurons where its activity is required for proper localization of G protein-coupled receptors to nonmotile primary cilia; tub-1 localizes predominantly to the cytoplasm, but undergoes transport supporting its involvement in neuronal trafficking and chemosensation.

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