E(z);trx

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Genetic mutants with E(z), trx alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Strain
E(z)[60];trx[B11]
Temperature °C
29
Diet
Media used for the life span assays contained 5% yeast, 10% sucrose, 5% cornmeal, 0.6% agar (wt/vol).
Lifespan (days)
39.0
Lifespan change (compared to wild type)
5.41%
Phenotype
E(z) 60 /+ mutants live 21% longer than Control whereas E(z) 60 trx B11 /+ + double mutants live 5% longer than Control. trx B11 /+ mutants themselves show no difference in median life span compared to Control.
Lifespan comparisons
Double mutant E(z)(df);trx(df) has a lifespan of 39.0 days, while single mutant trx(df) has a lifespan of 38.0 days, single mutant E(z)(df) has a lifespan of 44.0 days and wild type has a lifespan of 37.0 days.
Type of interaction
See methods
Dependent
Citation
View abstract
Siebold AP et al., 2010, Polycomb Repressive Complex 2 and Trithorax modulate Drosophila longevity and stress resistance. Proc Natl Acad Sci U S A. 107(1):169-74 20018689 Click here to select all mutants from this PubMed ID in the graph
Abstract
Polycomb Group (PcG) and Trithorax Group (TrxG) proteins are key epigenetic regulators of global transcription programs. Their antagonistic chromatin-modifying activities modulate the expression of many genes and affect many biological processes. Here we report that heterozygous mutations in two core subunits of Polycomb Repressive Complex 2 (PRC2), the histone H3 lysine 27 (H3K27)-specific methyltransferase E(Z) and its partner, the H3 binding protein ESC, increase longevity and reduce adult levels of trimethylated H3K27 (H3K27me3). Mutations in trithorax (trx), a well known antagonist of Polycomb silencing, elevate the H3K27me3 level of E(z) mutants and suppress their increased longevity. Like many long-lived mutants, E(z) and esc mutants exhibit increased resistance to oxidative stress and starvation, and these phenotypes are also suppressed by trx mutations. This suppression strongly suggests that both the longevity and stress resistance phenotypes of PRC2 mutants are specifically due to their reduced levels of H3K27me3 and the consequent perturbation of Polycomb silencing. Consistent with this, long-lived E(z) mutants exhibit derepression of Abd-B, a well-characterized direct target of Polycomb silencing, and Odc1, a putative direct target implicated in stress resistance. These findings establish a role for PRC2 and TRX in the modulation of organismal longevity and stress resistance and indicate that moderate perturbation of Polycomb silencing can increase longevity.

Strain
E(z)[60];trx[Z11]
Temperature °C
29
Diet
Media used for the life span assays contained 5% yeast, 10% sucrose, 5% cornmeal, 0.6% agar (wt/vol).
Lifespan (days)
42.0
Lifespan change (compared to wild type)
13.51%
Phenotype
E(z) 60 /+ mutants live 4.5% longer than E(z) 60 trx Z11 /+ double mutants. trx Z11 /+ mutants show no difference in median life span compared to Control.
Lifespan comparisons
Double mutant E(z)(df);trx(df) has a lifespan of 42.0 days, while single mutant trx(df) has a lifespan of 37.0 days, single mutant E(z)(df) has a lifespan of 45.0 days and wild type has a lifespan of 37.0 days.
Type of interaction
See methods
Dependent
Citation
View abstract
Siebold AP et al., 2010, Polycomb Repressive Complex 2 and Trithorax modulate Drosophila longevity and stress resistance. Proc Natl Acad Sci U S A. 107(1):169-74 20018689 Click here to select all mutants from this PubMed ID in the graph
Abstract
Polycomb Group (PcG) and Trithorax Group (TrxG) proteins are key epigenetic regulators of global transcription programs. Their antagonistic chromatin-modifying activities modulate the expression of many genes and affect many biological processes. Here we report that heterozygous mutations in two core subunits of Polycomb Repressive Complex 2 (PRC2), the histone H3 lysine 27 (H3K27)-specific methyltransferase E(Z) and its partner, the H3 binding protein ESC, increase longevity and reduce adult levels of trimethylated H3K27 (H3K27me3). Mutations in trithorax (trx), a well known antagonist of Polycomb silencing, elevate the H3K27me3 level of E(z) mutants and suppress their increased longevity. Like many long-lived mutants, E(z) and esc mutants exhibit increased resistance to oxidative stress and starvation, and these phenotypes are also suppressed by trx mutations. This suppression strongly suggests that both the longevity and stress resistance phenotypes of PRC2 mutants are specifically due to their reduced levels of H3K27me3 and the consequent perturbation of Polycomb silencing. Consistent with this, long-lived E(z) mutants exhibit derepression of Abd-B, a well-characterized direct target of Polycomb silencing, and Odc1, a putative direct target implicated in stress resistance. These findings establish a role for PRC2 and TRX in the modulation of organismal longevity and stress resistance and indicate that moderate perturbation of Polycomb silencing can increase longevity.

Search genes: E(z) trx

Entrez ID
Symbol
GenAge
FlyBase ID

39203
E(z)
View on GenAge (E(z))
FBgn0000629

Enhancer of zeste

Locus: Dmel_CG6502

Entrez ID
Symbol
GenAge
FlyBase ID

41737
trx
View on GenAge (trx)
FBgn0003862

trithorax

Locus: Dmel_CG8651

Orthologs of E(z);trx in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Mus musculus	Ezh1;Kmt2a
Mus musculus	Ezh2;Kmt2a

Orthologs of E(z) in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Caenorhabditis elegans	mes-2
Mus musculus	Ezh1
Mus musculus	Ezh2

Orthologs of trx in SynergyAge

Show in SynergyAge
Species	Gene

Not in SynergyAge
Species	Gene
Mus musculus	Kmt2a