old-1;rrf-3

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Genetic mutants with old-1, rrf-3 alterations

Names of genes are ordered alphabetically. For the order of interventions, please see the specific paper.

Temperature °C
20-25
Diet
OP50
Lifespan (days)
16.7
Lifespan comparisons
Double mutant old-1(RNAi);rrf-3(pk1426) has a lifespan of 16.7 days, while single mutant rrf-3(pk1426) has a lifespan of 15.0 days.
Citation
View abstract
Murphy CT et al., 2003, Genes that act downstream of DAF-16 to influence the lifespan of Caenorhabditis elegans. Nature. 424(6946):277-83 12845331 Click here to select all mutants from this PubMed ID in the graph
Abstract
Ageing is a fundamental, unsolved mystery in biology. DAF-16, a FOXO-family transcription factor, influences the rate of ageing of Caenorhabditis elegans in response to insulin/insulin-like growth factor 1 (IGF-I) signalling. Using DNA microarray analysis, we have found that DAF-16 affects expression of a set of genes during early adulthood, the time at which this pathway is known to control ageing. Here we find that many of these genes influence the ageing process. The insulin/IGF-I pathway functions cell non-autonomously to regulate lifespan, and our findings suggest that it signals other cells, at least in part, by feedback regulation of an insulin/IGF-I homologue. Furthermore, our findings suggest that the insulin/IGF-I pathway ultimately exerts its effect on lifespan by upregulating a wide variety of genes, including cellular stress-response, antimicrobial and metabolic genes, and by downregulating specific life-shortening genes.

Temperature °C
20-25
Diet
OP50
Lifespan (days)
17.6
Lifespan comparisons
Double mutant old-1(RNAi);rrf-3(pk1426) has a lifespan of 17.6 days, while single mutant rrf-3(pk1426) has a lifespan of 16.1 days.
Citation
View abstract
Murphy CT et al., 2003, Genes that act downstream of DAF-16 to influence the lifespan of Caenorhabditis elegans. Nature. 424(6946):277-83 12845331 Click here to select all mutants from this PubMed ID in the graph
Abstract
Ageing is a fundamental, unsolved mystery in biology. DAF-16, a FOXO-family transcription factor, influences the rate of ageing of Caenorhabditis elegans in response to insulin/insulin-like growth factor 1 (IGF-I) signalling. Using DNA microarray analysis, we have found that DAF-16 affects expression of a set of genes during early adulthood, the time at which this pathway is known to control ageing. Here we find that many of these genes influence the ageing process. The insulin/IGF-I pathway functions cell non-autonomously to regulate lifespan, and our findings suggest that it signals other cells, at least in part, by feedback regulation of an insulin/IGF-I homologue. Furthermore, our findings suggest that the insulin/IGF-I pathway ultimately exerts its effect on lifespan by upregulating a wide variety of genes, including cellular stress-response, antimicrobial and metabolic genes, and by downregulating specific life-shortening genes.

Search genes: old-1 rrf-3

Entrez ID
Symbol
GenAge
Wormbase ID

191737
old-1
View on GenAge (old-1)
WBGene00003862

Tyrosine-protein kinase receptor old-1

Locus: CELE_C08H9.5

Wormbase description: old-1 encodes a receptor protein tyrosine kinase; old-1 plays a role in stress resistance and regulation of adult lifespan; Murakami and Johnson showed that old-1 mRNA levels are upregulated in response to stress and in daf-2 and age-1 mutant backgrounds and that overexpression of old-1 in wild-type animals results in extended lifespan and increased resistance to heat and UV irradiation; in contrast, Murphy et al., showed that old-1 expression is downregulated in daf-2 mutants and that old-1(RNAi) in an rrf-3 mutant background slightly extends lifespan; an OLD-1::GFP fusion protein is expressed in the anterior region of worms, in neuronal, hypodermal, and pharyngeal tissues, as well as in the proximal region of the male gonad; expression is visible in young adults and appears to increase as animals age and in response to heat, starvation, or UV irradiation.

Entrez ID
Symbol
GenAge
Wormbase ID

174315
rrf-3
View on GenAge (rrf-3)
WBGene00004510

RNA-dependent RNA polymerase Family

Locus: CELE_F10B5.7

Wormbase description: rrf-3 encodes an RNA-directed RNA polymerase (RdRP) homolog that inhibits somatic RNAi, and thus promotes activity of repeated genes (e.g., multicopy transgenic arrays); the effect of RRF-3 on RNAi is opposite to that of RRF-1 (which stimulates somatic RNAi), which might arise from competition by RRF-3 with RRF-1 or EGO-1 in RNAi formation; rrf-3(allele) or rrf-3(allele2) mutants are hypersensitive to somatic RNAi, and conversely suppress the activity of an integrated rol6 (su1006) transgene.

Orthologs of old-1;rrf-3 in SynergyAge

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Species	Gene

Not in SynergyAge
Species	Gene

Orthologs of old-1 in SynergyAge

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Species	Gene

Not in SynergyAge
Species	Gene
Drosophila melanogaster	tor

Orthologs of rrf-3 in SynergyAge

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Species	Gene